47份外引小麦种质资源Waxy和HMW-GS分子检测与品质性状分析

为有效利用外引小麦种质资源,本研究对收集的47份外引小麦种质材料进行Waxy和HMW-GS等位基因的分子检测,并分析了其直链淀粉、支链淀粉、湿面筋等品质参数.结果 表明,在Wx-A1位点存在3种类型:Wx-A1a、Wx-A1g和Wx-A1b,39份材料(82.98％)为Wx-A1a类型;Wx-B1位点3种类型:Wx-B...

Molecular Detection and Quality Properties Analysis of Waxy and HMW-GS in 47 Introduced Wheat Germplasms

In order to effectively utilize wheat germplasm introduced from abroad,Waxy and HMW-GS alleles were detected in 47 introduced wheat materials,and the quality parameters of amylose,amylopectin and wet gluten were analyzed.The results showed that there were three types of Wx-A1 a,Wx-A1 g and Wx-A1 b at the Wx-A1 locus,and 39 materials(82.98%)were mainly of Wx-A1 a type.Wx-B1 a,Wx-B1 e and Wx-B1 b were located in the Wx-B1 locus,and 37 materials(78.72%)were mainly of Wx-B1 a type.Wx-D1 a and Wx-D1 b were discovered at Wx-D1 locus,and 46 materials were of Wx-D1 a type.A total of 8 Wx-1 allele combinations were identified,and 31 materials(65.96%)were mainly of Wx-A1 a/B1 a/D1 a combination.The results also showed that the Glu-A1 alleles Ax2^*,Null and Ax1 were distributed in 18,18 and 11 materials,respectively.At the Glu-D1 locus,it was found that 23(48.94%)and 21(44.68%)materials contained Dx2 and Dx5,respectively;22(46.81%)and 20(42.55%)materials contained Dy12 and Dy10,respectively;2 materials possessed heterozygous Dy10+Dy12 type.A total of 19 kinds of Glu-A1/D1 allele combinations were identified,and Null/Dx5+Dy12 allele combinations were displayed in 7 materials.The quality analysis showed that the materials with Wx-A1 a/B1 a/D1 a had relatively high amylose and low amylopectin contents,and the materials with Ax1 or Ax2^*and Dx5+Dy10 possessed relatively high wet gluten content.Those results suggested that the introduced wheat germplasms were rich in Waxy and HMW-GS alleles,which would provide reference for rational utilization of germplasm resources and quality improvement of modern common wheat.