发菜PspA基因克隆及其转基因植物的抗旱性分析

为探讨发菜噬菌体休克蛋白A(PspA)的分子信息和基因功能,本研究通过设计特异引物克隆发菜PspA基因,采用qRT-PCR技术,分析发菜PspA基因在干旱胁迫下的表达模式;构建PspA真核表达载体pCAM35 s-GFP-PspA,对PspA进行亚细胞定位和PspA基因拟南芥遗传转化,并对阳性转化拟南芥分别进行Sout...

Isolation of the PspA Gene from Nostoc flagelliforme and Analysis of Its Drought Resistance in Transgenic Plants

In order to study the functional basis of Phage shock protein A(PspA),the full-length coding sequence(CDS)of the PspA gene was isolated based on sequence homolog and its expression pattern of PspA from Nostoc flagelliforme Born.&Flah.under drought stress was studied by RT-qPCR.We further generated a plasmid pCAM35 s-GFP-PspA which were subjected for a subcellular localization analysis and a transformation in Arabidopsis thaliana(L.)Heynh.PspA contained a 777 bp full-length coding sequence,and this gene was significantly up-regulated in N.flagelliforme Born.&Flah.upon drought stress treatment.A transient expression of PspA in N.benthamiana Domin suggested a sublocalization on the plasma membrane.Moreover,by transforming this gene in A.thaliana(L.)Heynh.,the transgenic plants showed low copies of the PspA gene using Southern hybridization and expressed the recombination protein by Western blot analysis.The transgenic plants expressing the PspA gene,if compared to the wild type plants,were found to be highly resistant against drought stress.Collectively,the results provided a foundation for further exploring the biological function of the N.flagelliforme Born.&Flah.PspA gene that interplays with drought stress.