Monodispersity of recombinant Cre recombinase correlates with its effectiveness in vivo |
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| Authors: | Paola Capasso Marisa Aliprandi Giuseppe Ossolengo Frank Edenhofer Ario de Marco |
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| Institution: | (1) Cogentech, Consortium for Genomics Technologies, via Adamello 16, 20139 Milan, Italy;(2) Stem Cell Engineering Group, Institute of Reconstructive Neurobiology, University of Bonn, Life & Brain Center and Hertie Foundation Sigmund-Freud-Strasse 25, D-53105 Bonn, Germany |
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| Abstract: | Background Cre recombinase is a common reagent used for the in vivo on/off switching of the expression of target genes flanked by loxP sites. In particular, recombinant TAT-Cre fusion constructs purified from bacteria have been used to promote the cell uptake
of the enzyme. However, the recovery of active TAT-Cre remains a demanding process and its specific activity varies significantly
among batches, making difficult data comparison. |
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