PCIB an Antiauxin Enhances Microspore Embryogenesis in Microspore Culture of Brassica juncea |
| |
| Authors: | Pradeep K. Agarwal Parinita Agarwal Jan B. M. Custers Chun-ming Liu S. S. Bhojwani |
| |
| Affiliation: | (1) Department of Botany, University of Delhi, Delhi, 110 007, India;(2) Department of Life Sciences, Bhavnagar University, Bhavnagar, 364 002, India;(3) Plant Research International, Wageningen University and Research Centre, 6700 AA Wageningen, The Netherlands;(4) Present address: Marine Algae and Marine Environment, Central Salt and Marine Chemicals Research Institute, Bhavnagar, 364 002, India;(5) Present address: Dayalbagh Educational Institute, Dayalbagh, Agra, 282 005, India |
| |
| Abstract: | An efficient protocol to improve microspore embryogenesis is established in an important oleiferous crop, Brassica juncea (Indian mustard). Colchicine was used for enhancing microspore embryogenesis and also to obtain doubled haploid embryos. Colchicine at high concentrations (>10 mg l−1), for 24 h, proved convenient for direct recovery of diploid embryos. Higher temperature treatment and an antiauxin PCIB (p-chlorophenoxyisobutyric acid) enhanced microspore embryogenesis significantly as compared to colchicine. An increase in temperature from 32°C to 35°C proved very efficient in increasing embryogenesis by 10-fold. The highest embryogenesis rate was obtained when PCIB was added at 35°C in the culture after 1 day of culture initiation. 20 μM PCIB could enhance microspore embryogenesis by 5-fold. Different abnormal shapes of embryos like lemon, banana, flask and fused cotyledons were observed. Both normal and fused cotyledonous embryos showed normal germination when transferred on the B5 basal medium. |
| |
| Keywords: | Antiauxin Brassica juncea High temperature Microspore embryogenesis |
| 本文献已被 SpringerLink 等数据库收录！ |
|
