A model for mercuric ion reduction in recombinant Escherichia coli |
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Authors: | Philippidis G P Schottel J L Hu W S |
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Affiliation: | Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, Minnesota 55455. |
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Abstract: | Plasmid-encoded mercuric reduction involves transfer of Hg(2+) across the cellular envelope and reduction to Hg(0) by the cytoplasmic mercuric reductase using NADPH. A mathematical model was developed for the binding and transfer of Hg(2+) by transport proteins and the subsequent reduction of Hg(2+). The values of the model parameters were determined using experimental data. The derived rate expressions were similar to the previously experimentally determined ones. The model predicted that a differential amplification of the transport protein relative to mercuric reductase expression levels may enhance the Hg(2+) reduction rate in whole cells. |
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