Calcium requirements of residual protease in Bacillus subtilis DB104 |
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Authors: | A Longo J R Snay L Zimmer M M Ataai |
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Institution: | (1) Department of Chemical and Petroleum Engineering, University of Pittsburgh, 15261 Pittsburgh, PA, USA |
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Abstract: | Summary
Bacillus subtilis DB104, a double mutant which does not synthesize neutral or alkaline proteases, was shown to exhibit some residual proteolytic activity when grown in both batch and continuous cultures. A major protein component responsible for about 70% of extracellular residual protease activity was reversibly deactivated by removal of calcium. |
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