缩胆囊素、胃泌素及丙谷胺对大鼠胆汁分泌的影响

本文旨在研究五肽胃泌素(P-Gas)、缩胆囊素(CCK)与其受体拮抗剂丙谷胺(PGM)单独静脉输注或联合应用对大鼠胆汁流量及胆汁成分排泌量的影响。结果表明:(1)P-Gas无利胆作用;(2)CCK-8仅少量增加胆汁及 HCO_3~-的分泌量;(3)PGM 明显地增加胆汁及胆汁中 HCO_3~-和 CI~-的排泌量,但胆酸分泌量不增加;(4)当 CCK-8 2.3μg/(kg·h)静脉灌注与 PGM 200mg 灌胃联合应用时,胆汁、HCO_3~-和 CI~-的排泌量进一步增加,但P-Gas 2μg/(kg·h)与 PGM 联合应用时的胆汁排泌量低于单独应用 PGM 组。以上结果证明:PGM 有明显的促胆汁分泌作用,其机制属于非胆酸依赖性利胆、而CCK-8只是胆汁分泌的微弱刺激剂,P-Gas 则无利胆作用。在 CCK-8与 PGM 二组间,似有促胆汁分泌的协同作用;但表现在 P-Gas 与 PGM 二组间的作用,似为一种拮抗作用。

EFFECTS OF CHOLECYSTOKININ,GASTRIN AND PROGLUMIDE ON BILE SECRETION IN RATS

The aim of this study is to observe the effects of pentagastrin (P-Gas), CCK-8 and proglumide (PGM), which were used solely or in combination on choleresis in rats prepared with neck vein and hepatic-bile duct intubation. The results indicate that: 1) P-Gas does not provide cholecystic effect; 2) CCK-8 has a little stimulating effect on bile flow and output of bicarbonate; 3) PGM increases the bile flow and the outputs of biliary bicarbonate and chloride significantly, but it can not increase the bile acid output; 4) after administration of CCK-8 2.3 micrograms/(kg.h) iv infusion and PGM 200 mg ig in combination the outputs of bile flow, bicarbonates and chloride were greater than to PGM given alone, but after P-Gas 2 micrograms/(kg.h) was used together with PGM the outputs were less than to PGM given alone. The results suggest that PGM has a remarkable effect of choleresis and its mechanism may be attributed to bile acid independent fraction, while CCK-8 was only a weaker stimulant and P-Gas can not modify the bile flow and bile components. In addition, there may be a synergism effect between PGM and CCK-8 and an antagonist suppression between PGM and P-Gas on choleresis in the rat.