Integrated diversity analysis of the microbial community in a reverse osmosis system from a Brazilian oil refinery |
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| Authors: | Daiane R.B. Belgini Virgínia M. Siqueira Douglas M. Oliveira Samantha G. Fonseca Viviane Piccin-Santos Roberto S. Dias Larissa Quartaroli Rodrigo S. Souza Ana Paula R. Torres Maíra P. Sousa Cláudio M. Silva Cynthia C. Silva Sérgio O. De Paula Valéria M. Oliveira |
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| Affiliation: | 1. Institute of Biology, University of Campinas – UNICAMP, P.O. Box 6109, 13083-970, Campinas, SP, Brazil;2. Microbial Resources Division, Research Center for Chemistry, Biology and Agriculture (CPQBA), Campinas University – UNICAMP, P.O. Box 6171, 13081-970, Campinas, SP, Brazil;3. General Biology Department, Federal University of Viçosa – UFV, 36570-000, Viçosa, MG, Brazil;4. Building Engineering Department, Federal University of Viçosa – UFV, 36570-000, Viçosa, MG, Brazil;5. PETROBRAS Research and Development Center (CENPES), Biotechnology Management, Av. Horácio Macedo, 950, Expansão, Ala C, 21941-915, Ilha do Fundão, Rio de Janeiro, RJ, Brazil;6. Microbiology Department, Federal University of Viçosa – UFV, 36570-000, Viçosa, MG, Brazil |
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| Abstract: | Oil refineries are known for the large volume of water used in their processes, as well as the amount of wastewater generated at the end of the production chain. Due to strict environmental regulations, the recycling of water has now become a viable alternative for refineries. Among the many methods available to treat wastewater for reuse, the use of membranes in reverse osmosis systems stands out due to several economic and environmental benefits. However, these systems are vulnerable to contamination and deposition of microorganisms, mainly because of the feedwater quality. In this study, the microbial diversity of feedwater and reverse osmosis membranes was investigated using a combination of culture-dependent and culture-independent methods in order to characterize the microorganisms colonizing and deteriorating the membranes. In total, 37 bacterial isolates, 17 filamentous fungi and approximately 400 clones were obtained and analyzed. Among the bacterial genera identified, the most represented were Sphingobium, Acidovorax, Microbacterium, Rhizobium and Shinella. The results revealed genera that acted as candidate key players in initial biofilm formation in membrane systems, and provided important information concerning the microbial ecology of oligotrophic aquatic systems. |
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| Keywords: | Microbial ecology Reverse osmosis system Oil refinery Biofilm Biofouling |
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