Phenotypic and genetic diversity of Moroccan rhizobia isolated from Vicia faba and study of genes that are likely to be involved in their osmotolerance |
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Authors: | Loubna Benidire Majida Lahrouni Khalid Daoui Zain el Abidine Fatemi Ricardo Gomez Carmona Michael Göttfert Khalid Oufdou |
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Institution: | 1. Laboratory of Biology and Biotechnology of Microorganisms, Faculty of Sciences Semlalia, Cadi Ayyad University, PO Box 2390, Marrakech, Morocco;2. Technische Universität Dresden, Institut für Genetik, Helmholtzstr. 10, D-01069 Dresden, Germany;3. Centre Régional de la Recherche Agronomique de Meknès, INRA, Morocco;4. Department of Biology, Faculty of Science and Techniques, BP. 509, 52000 Boutalamine, Errachidia, Morocco |
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Abstract: | Rhizobia are symbiotic nitrogen-fixing bacteria in root nodules of legumes. In Morocco, faba bean (Vicia faba L.), which is the main legume crop cultivated in the country, is often grown in marginal soils of arid and semi-arid regions. This study examines the phenotypic diversity of rhizobia nodulating V. faba isolated from different regions in Morocco for tolerance to some abiotic stresses. A total of 106 rhizobia strains isolated from nodules were identified at the species level by analysing 16S rDNA. Additionally, for selected strains recA, otsA, kup and nodA fragments were sequenced. 102 isolates are likely to belong to Rhizobium leguminosarum or R. laguerreae and 4 isolates to Ensifer meliloti. All strains tolerating salt concentrations of 428 or 342 mM NaCl as well as 127 or 99 mM Na2SO4 were highly resistant to alkaline conditions (pH 10) and high temperature (44 °C). Three strains: RhOF4 and RhOF53 (both are salt-tolerant) and RhOF6 (salt-sensitive) were selected to compare the influence of different levels of salt stress induced by NaCl on growth and on trehalose and potassium accumulation. We find a direct correlation between the trehalose contents of the rhizobial strains and their osmotolerance. |
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Keywords: | Rhizobia Osmotolerance Trehalose Potassium |
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