Lectin levels in tissues of cultured immature wheat embryos |
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Authors: | P C Morris S E Maddock M G K Jones D J Bowles |
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Institution: | (1) Department of Biochemistry, University of Leeds, LS2 9JT Leeds, UK;(2) Department of Biochemistry, Rothamsted Experimental Station, AL5 2JQ Harpenden, Herts., UK |
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Abstract: | Levels of wheat germ agglutinin have been determined by radioimmunoassay in tissues of immature wheat embryos cultured under different conditions in order to determine the suitability of the lectin as a marker for somatic embryogenesis. Embryos cultured on media favouring continued embryo development accumulated lectin in a similar manner to zygotic embryos in planta unless precocious germination occurred. Embryos cultured on media containing 2,4-D produced callus, and some of this developed somatic embryos. Both embryogenic and non-embryogenic callus contained WGA, that in non-embryogenic callus possibly arising from developmentally arrested root primordia.Abbreviations ABA
abscisic acid
- dpa
days post anthesis
- PBS
phosphate buffered saline, (10 mM KH2PO4 K2HPO4, 145 mM NaCl, pH 7.4)
- RIA
radioimmunoassay
- WGA
wheat germ agglutinin
- 2,4-D
2,4-dichlorophenoxyacetic acid |
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Keywords: | |
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