Inhibition of the elongation step of protein synthesis by vaccinia virus |
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Authors: | P W Tas O H Martini |
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Institution: | Institute for Virology and Immunology, University of Würzburg, Versbacher Straβe 7, D-8700 Würzburg, FRG |
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Abstract: | Vaccinia cores inhibit translation in cell-free protein synthesis systems at two stages: initiation; and, as shown here, elongation. The former effect tends to obscure the latter. Elongation control could, however, be revealed as follows: when, in a reticulocyte of L-cell lysate, initiation was blocked by a drug (edein), the residual 35S]methionine incorporation was severely reduced by the subsequent addition of vaccinia cores. The elongation block could also be demonstrated by analysis of ribosome profiles: treatment with edein alone permitted ribosomal run-off; treatment with either the elongation inhibition anisomycin or with cores preserved the polyribosomes. |
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Keywords: | Vaccinia core Control of protein synthesis Cell-free translation system Elongation inhibition |
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