Cohesin axis maturation and presence of RAD51 during first meiotic prophase in a true bug |
| |
Authors: | Alberto Viera Juan Luis Santos María Teresa Parra Adela Calvente Rocío Gómez Roberto de la Fuente José Ángel Suja Jesús Page Julio S. Rufas |
| |
Affiliation: | (1) Departamento de Biología, Edificio de Biológicas, Facultad de Ciencias, Universidad Autónoma de Madrid, 28049 Madrid, Spain;(2) Departamento de Genética, Facultad de Biología, Universidad Complutense de Madrid, 28040 Madrid, Spain;(3) Unidad de Biología Celular, Departamento de Biología, Facultad de Ciencias, Edificio de Ciencias Biológicas, Universidad Autónoma de Madrid, C/Darwin 2, 28049 Madrid, Spain |
| |
Abstract: | We have analyzed in a true bug, Graphosoma italicum (Pentatomidae, Hemiptera), the temporal and functional relationships between recombination events, synapsis progression, and SMC1α and SMC3 cohesin axis maturation throughout the male first meiotic prophase. The localization of the histone variant histone H3 trimethylated at lysine 9 at chromosome ends has allowed us to determine the association of these heterochromatic domains through prophase I stages. Results highlighted that cohesins provide to be good markers for synapsis progression since the formation, morphology, and development of the SMC1α and SMC3 cohesin axes resemble the synaptonemal complex dynamics and, also, that in this species the initiation of recombination precedes synapsis. In addition, we have carried out an accurate cytological characterization of the diffuse stage, which takes place after pachytene, and also analyzed the presence of the cohesin subunits, SMC1α and SMC3, and the recombinase RAD51 at this stage. The mechanisms underlying the absence of SMC1α and SMC3 axes from the diffuse stage onwards are discussed. Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|