Efficient ligation and cloning of DNA fragments with 2-bp overhangs |
| |
Authors: | Rajiv Kumar Ranjan |
| |
Affiliation: | Institute of Microbial Technology, Sector-39a, Chandigarh 160036, India1A Constituent Establishment of the Council of Scientific and Industrial Research (CSIR).1 |
| |
Abstract: | Various methods of ligation are currently available and routinely used by molecular biologists, such as blunt end ligation, cohesive end (two and four overhangs), and ligation of Taq polymerase-derived products. However, there is no efficient method for the cloning of DNA fragments with 2-bp overhangs. We present a simple method for the efficient ligation of DNA fragments with 2-bp overhanging ends, ranging in size from 0.7 to 2.5 kbp. Our method involves the initial heating and flash freezing of the vector-insert DNA mix, and a subsequent unique ligation reaction. This method provides a new molecular biology tool for researchers. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|