Determination of thermodynamic parameters of cadmium(II) association to glutathione using the fluorescent reagent FluoZin-1 |
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Authors: | Shinya Ogawa |
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Institution: | Department of Applied Biological Chemistry, University of Tokyo, Yayoi, Bunkyo, Tokyo 113-8657, Japan |
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Abstract: | A method was developed to determine the conditional association constants of cadmium(II) Cd(II)] complexes based on the reagent FluoZin-1, which forms a fluorescent complex with Cd(II). A solution containing Cd(II) and FluoZin-1 was titrated with glutathione while determining fluorescence intensity of FluoZin-1 to estimate levels of free Cd(II). The results were analyzed with a nonlinear least-squares method using the Solver algorithm of Microsoft Excel to yield conditional association constants for 1:1 and 1:2 Cd(II)-glutathione complexes. The values obtained were consistent with those reported previously using isothermal titration calorimetry. |
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Keywords: | Association constant Cadmium FluoZin-1 Glutathione |
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