Modified nano-TiO2 coupled with fluorescence spectroscopy for the separation/analysis of l-tryptophan |
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Authors: | Xiashi Zhu Ying Gu |
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Affiliation: | Department of Chemistry, Yangzhou University, Yangzhou 225002, People’s Republic of China |
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Abstract: | A novel adsorbent of carboxymethyl-β-cyclodextrin modified nanometer TiO2 (CM-β-CD/TiO2) was prepared and used as a solid-phase extraction (SPE) material coupled to fluorescence spectroscopy determination of l-tryptophan (l-Trp) in biological samples. The experimental conditions for modified nanometer TiO2 separation/preconcentration of l-Trp were optimized. The adsorption capacity of CM-β-CD/TiO2 for l-Trp was 75.2 μg/g. The linear range, detection limit (DL), and the relative standard deviation (RSD) were 0.10-1.20 μg/mL, 18.8 ng/mL, and 0.67% (n = 3, 1.0 μg/mL), respectively, with a preconcentration factor of 10. The developed method was applied to determination of l-Trp in real samples and the recoveries were found to be in the range of 99.2-100.3%. For validation, a comparison material of NIC-140686 sample was analyzed and the determined value was in good agreement with the certified value. |
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Keywords: | smallcaps" >l-Tryptophan Carboxymethyl-β-cyclodextrin Nanometer titanium dioxide Fluorescence spectroscopy Solid-phase extraction |
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