Production of prostacyclin, 6-keto-PGF1α and thromboxane B2 by incubated samples of umbilical vessels: A comparison of methods for expressing the reults on dry weight, wet weight, protein or DNA bases |
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| Authors: | C. Benedetto L. Rey A.M. Tavella E. Petitti M. Barbero M. Massobrio T.F. Slater |
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| Affiliation: | 1. Institute of Obstetrics and Gynaecology, University of Turin, Via Ventimiglia 3, 10126 Torino, Italy;2. Biochemistry Department, Brunel University, Uxbridge, UB8 3PH Middx., U.K. |
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| Abstract: | The production of PG12 (determined by abioassay), and of 6-keto-PGF1α and TXB2 (determined by radioummunoassay) by samples of human umbilical vessels have been measured. The results have been calculated on four bases: dry weigt, wet weight, protein and DNA.There was a higher production of PG12 and 6-keto-PGF1α by umbilical veins than by umbilical arteries; no significant difference in TXB2 production was observed between umbilical veins and arteries. The ratio of 6-keto-PGF1α: TXB2 production was about 100 for the samples of veins and about a40 for the samples of arteries.The best methods of expressing the results were on the bases of protein and DNA, the latter basis being marginally the best. The least satisfactory method for expressing the results was that based on dry weight.The physiological and practical implications of the results are discussed. |
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