The effect of actidione on the regeneration of yeast protoplasts |
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| Authors: | L. Sošková A. Svoboda J. Soška |
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| Affiliation: | (1) Department of General Biology, Faculty of Medicine, the University of Jan Evangelista Purkyně, Brno;(2) Present address: Institute of Biophysics, Czechoslovak Academy of Sciences, Brno |
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| Abstract: | InSaccharomyces cerevisiae, actidione (cycloheximide) at a concentration of 10 μg/ml inhibits protein synthesis, cell multiplication and regeneration of protoplasts to normal cells. Resistance of cells to actidione is not determined by properties of the cell wall. The effect of actidione is rather cytostatic than cytocidal. The ability of protoplasts to regenerate is irreversibly blocked after more than 2 h incubation with actidione. |
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