Organ culture passage enhances the oncogenicity of carcinogen-induced hyperplastic mammary nodules |
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Authors: | E M Rivera S D Hill M Taylor |
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Institution: | (1) Department of Zoology, Michigan State University, 48824 East Lansing, Michigan |
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Abstract: | Summary The purpose of this study was to determine whether culturing hyperplastic mammary nodules in hormone-free medium would enhance
their oncogenicity following subsequent transplantation into mammary fat pads. The underlying hypothesis is that the proliferation
of transformed cells within the nodules is inhibited by hormone-dependent normal cells also present in the nodules. Accordingly,
both primary hyperplastic nodules and tissues from a hyperplastic outgrowth of a primary nodule were maintained as organ cultures
for varying periods in hormone-free Medium 199. The results show that whereas noncultured nodules developed mammary tumors
at an incidence of only 15%, those passaged in organ culture gave rise to mammary tumors at an incidence of 40 to 43%. This
threefold enhancement in the oncogenicity of mammary nodules is interpreted to be due, at least in part, to a reduction in
the normal mammary cell content of nodules. Consistent with this interpretation is the observation that cultured nodules gave
rise to mammary outgrowths that were predominantly hyperplastic, whereas noncultured nodules generated outgrowths with varying
proportions of hyperplastic and normal ductal mammary tissue.
This investigation was supported by National Cancer Institute Grant CA-17862. |
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Keywords: | mammary hyperplasia nodule organ culture enhanced oncogenicity transplantation |
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