Sucrose-Phosphate Synthase from Synechocystis sp. Strain PCC 6803: Identification of the spsA Gene and Characterization of the Enzyme Expressed in Escherichia coli |
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| Authors: | Leonardo Curatti Eduardo Folco Paula Desplats Gustavo Abratti Vernica Limones Luis Herrera-Estrella and Graciela Salerno |
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| Institution: | Leonardo Curatti, Eduardo Folco, Paula Desplats, Gustavo Abratti, Verónica Limones, Luis Herrera-Estrella, and Graciela Salerno |
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| Abstract: | The first identification and characterization of a prokaryotic gene (spsA) encoding sucrose-phosphate synthase (SPS) is reported for Synechocystis sp. strain PCC 6803, a unicellular non-nitrogen-fixing cyanobacterium. Comparisons of the deduced amino acid sequence and some relevant biochemical properties of the enzyme with those of plant SPSs revealed important differences in the N-terminal and UDP-glucose binding site regions, substrate specificities, molecular masses, subunit compositions, and regulatory properties. |
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