Galactose oxidase production by immobilized cells ofDactylium dendroides |
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| Authors: | M. Ŝůchová V. Křen K. Demnerová T. Macek |
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| Affiliation: | (1) Dept. of Biochemistry and Microbiology, Institute of Chemical Technology, 166 28 Prague 6, Czechoslovakia;(2) Institute of Microbiology, Cz. Acad. Sci., 142 20 Prague 4, Czechoslovakia;(3) Institute of Organic Chemistry and Biochemistry, Cz. Acad. Sci., 166 10 Prague 6, Czechoslovakia |
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| Abstract: | Summary Dactylium dendroides cells were immobilized with calcium alginate, calcium pectate and k-carrageenan. Alginate immobilized cells produced relatively small amounts of (D-galactose: O2 oxidoreductase, EC 1.1.3.9, GOase). Pectate immobilized cells gave the best yield of GOase, which was comparable with that obtained with free cells, and productivity could be extended up to 28 days (7 cycles). Controlled dosage of phosphate to the medium markedly improved GOase production with higher yields per cycle than with free cells. |
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