Simultaneous detection of seven mutations with seven forward primers and one common reverse primer in a single PCR step |
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| Authors: | Wang Qiang Liu Qiuyun Li Bao-jian |
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| Affiliation: | Biotechnology Research Center and Key Laboratory of Gene Engineering of Ministry of Education, School of Life Sciences, Zhongshan University, Canton 510275, PR China. |
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| Abstract: | A new PCR method is described here to amplify simultaneously several fragments sharing the same template in a single PCR step with a hemi-primer. Using this method, we successfully detected various point mutations in tissue plasminogen activator (tPA) mutants. |
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