Native subtilisin Karlsberg and modified subtilisin 72 as effective catalysts of peptide bond formation in organic media |
| |
| Authors: | O M Anikina T A Semashko E S Oksenoit E N Lysogorskaya I Yu Filippova |
| |
| Institution: | (1) Faculty of Bioengineering and Bioinformatics, Moscow State University, Vorob’evy gory, Moscow, 119992, Russia;(2) Faculty of Chemistry, Moscow State University, Vorob’evy gory, Moscow, 119992, Russia |
| |
| Abstract: | The activity and stability of native subtilisin Karlsberg and subtilisin 72 and their complexes with sodium dodecyl sulfate (SDS) in organic solvents were studied. The kinetic constants of the hydrolysis of specific chromogenic peptide substrates Z-Ala-Ala-Leu-pNA and Glp-Ala-Ala-Leu-pNA by the subtilisins were determined. It was found that the subtilisin Karlsberg complex with SDS in anhydrous organic solvents is an effective catalyst of peptide synthesis with multifunctional amino acids in positions P 1 and P 1 ′ (Glu, Arg, and Asp) containing unprotected side ionogenic groups. |
| |
| Keywords: | enzymatic peptide synthesis organic solvents SDS-subtilisin Karlsberg comple |
| 本文献已被 SpringerLink 等数据库收录！ |
|
