Cleavage at 5-methylcytosine in DNA by photosensitized oxidation with 2-methyl-1,4-naphthoquinone tethered oligodeoxynucleotides |
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| Authors: | Yamada Hisatsugu Tanabe Kazuhito Nishimoto Sei-Ichi |
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| Affiliation: | Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Kyoto 615-8510, Japan. |
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| Abstract: | Photosensitized one-electron oxidation of 5-methylcytosine in DNA by 2-methyl-1,4-naphthoquinone, attached to 5'-end of an oligodeoxynucleotide strand, produced 5-formylcytosine and led to selective DNA strand cleavage at the original 5-methylcytosine configuration. This specified photoreaction is useful for positive display of 5-methylcytosine in DNA on a sequencing gel. |
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