首页 | 本学科首页   官方微博 | 高级检索  
   检索      


In vitro antileishmanial activity and cytotoxicity of essential oil from Lippia sidoides Cham
Authors:de Medeiros Maria das Graças Freire  da Silva Aline Caroline  Citó Antonia Maria das Graças Lopes  Borges Andrezza Raposo  de Lima Sidney Gonçalo  Lopes José Arimatéia Dantas  Figueiredo Regina Celia Bressan Queiroz
Institution:aPrograma de Pós-graduação em Biotecnologia-RENORBIO, Universidade Federal do Piaui, Brazil;bDepartamento de Microbiologia, Centro de Pesquisa Aggeu Magalhães, FIOCRUZ, PE, Av. Prof. Moraes Rego s/n Cidade Universitária-Campus da UFPE, Recife, PE, 50670-420, Brazil;cDepartamento de Química, Universidade Federal do Piaui, Campus Universitário Ministro Petrônio Portela-Ininga, Teresina, PI, 64049-550, Brazil;dPrograma de Pós-graduação em Saúde Pública, Centro de Pesquisas Aggeu Magalhães, FIOCRUZ, Brazil
Abstract:Leishmaniasis is a widespread tropical infection caused by different species of Leishmania protozoa. There is no vaccine available for Leishmania infections and conventional treatments are very toxic to the patients. Therefore, antileishmanial drugs are urgently needed. In this study we have analyzed the effects of essential oils from Lippia sidoides (LSEO) and its major compound thymol on the growth, viability and ultrastructure of Leishmania amazonensis. The essential oil and thymol showed significant activity against promastigote forms of L. amazonensis, with IC50/48 h of 44.38 and 19.47 μg/mL respectively. However, thymol showed toxicity against peritoneal macrophages and low selectivity against the promastigotes when compared with the crude LSEO. On the other hand, no cytotoxic effect was observed in macrophages treated with the crude essential oil. Incubation of L. amazonensis-infected macrophages with LSEO showed a marked reduction in amastigote survival within the macrophages. Significant morphological alterations as accumulation of large lipid droplets in the cytoplasm, disrupted membrane and wrinkled cells were usually seen in treated parasites. The LSEO's activity against both promastigote and the amstigote forms of L. amazonensis, together with its low toxicity to mammalian cells, point to LSEO as a promising agent for the treatment of cutaneous leishmaniasis.
Keywords:Lippia sidoides  Essential oil  Antileishmanial activity Leishmania amazonensis  Ultrastructure
本文献已被 ScienceDirect PubMed 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号