| 棉铃虫中肠几种酶的组织化学研究 |
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| 引用本文： | 李殿香,吴中华.棉铃虫中肠几种酶的组织化学研究[J].昆虫知识,1999,36(2):78-80. |
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| 作者姓名： | 李殿香 吴中华 |
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| 作者单位： | [1]济宁师范专科学校化学系 [2]黄岗师范专科学校生物系 |
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| 基金项目： | 国家教育部优秀年轻教师基金，国家自然科学基金 |
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| 摘 要： | 采用酶组织化学方法研究了棉铃虫中肠三磷酸腺苷酶、酸性磷酸酶和碱性磷酸酶的分布和活性。结果显示三种酶在棉铃虫中肠均有分布，但分布部位各不相同，其中，三磷酸腺苷酶在肠壁分泌细胞、肠壁细胞、环肌和纵肌均有分布，以肠壁分泌细胞活性较高；碱性磷酸酶主要分布于肠壁分泌细胞，在肠壁分泌细胞做绒毛处活性最高；酸性磷酸酶分布于肠壁细胞、环肌和纵肌，在肠壁细胞底膜处活性最高。这些酶可以作为棉铃虫中肠不同条件下的生理指标。
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| 关 键 词： | 棉铃虫 酸性磷酸酶 碱性磷酸酶 三磷酸腺苷酶 |
Histochemistry of the enzymes from midgut of cotton bollworm Helicoverpa armigera (Hiibner). |
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| LI Dian-Xiang.Histochemistry of the enzymes from midgut of cotton bollworm Helicoverpa armigera (Hiibner).[J].Entomological Knowledge,1999,36(2):78-80. |
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| Authors: | LI Dian-Xiang |
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| Abstract: | The microstructure of midgut of cotton bollworm,Helicoverpa armigera (Hubner)has been observed. The distribution of adenosine triphosphatase, alkaline phosphatase and acid phosphatase on the midgut of the insect has been investigated by methods of enzymological histochemistry. The results show that there are three enzymes distributed on the midgut. Their location, however, is different. Adenosine triphosphatase is widely distributed on secretive epithelial cells, longitudinal muscle and circular muscle . Alkaline phosphatase is distributed on secretive epithelial cells. Acid phosphatase is distributal around bottom membrane, circular muscle, circtilar membrane and longitudinal muscle. These enzymes can be used as indicators of the physiological condition of the midgut. |
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| Keywords: | Helicoverpa armigera (Hubner) adenosine triphosphatase alkaline phosphatase acid phosphatase |
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