Analysis of inactive renin by renin profragment monoclonal antibodies |
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Authors: | I Gaillard J A Fehrentz D Simon G Badouaille R Seyer B Castro B Pau P Corvol |
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Affiliation: | 1. INSERM U36, 17 rue du Fer-á-Moulin, 75005 Paris, France;2. Centre CNRS-INSERM de Pharmacologie Endocrinologie, Rue de la Cardonille, 34033 Montpellier, France and Centre de Recherche Clin-Midy SANOFI, Rue du Professeur Blayac, 34100 Montpellier, France |
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Abstract: | Two peptides were synthesized, corresponding to the sequences (-19 to -7) and (-26 to -17) of the prorenin prosegment. Monoclonal antibodies were raised to these sequences and used to characterize human plasma inactive renin. Only anti (-19 to -7) reacted with inactive renin, as measured by direct assay or affinity chromatography. The data were used to evaluate two possible inactive renin stuctures: plasma inactive renin is a truncated prorenin lacking the prosegment N-terminal portion; its spatial conformation masks the N-terminal extremity, preventing interaction of this region with specific antibodies. |
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