Effect of DTNB on glutamate dehydrogenase activity in root and shoot extracts of maize seedlings |
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Authors: | R S Sengar H S Srivastava |
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Institution: | 1. Department of Plant Science, Rohilkhand University, 243006, Bareilly, India
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Abstract: | NADH specific glutamate dehydrogenase (GDH) activity was examined in roots and shoots of maize seedlings grown in half-strength
Hoagland’s solution containing NH4NO3 as sole nitrogen source under irradiance of 60 W m−2 and temperature of 25±2°C. When 5,5′-dithio-bis (2-nitrobenzoic acid) (DTNB) was supplied to the assay mixture, it inhibited
NADH-GDH activity in both roots and shoots, irrespective of whether the enzymes were extracted from light- or dark-treated
roots and shoots. In each case the inhibition increased with the increase in DTNB concentration. At the maximum concentration
of DTNB used (20 μM) the inhibition of shoot NADH-GDH was more pronounced than inhibition of root enzyme. This indicated differences
in shoot and root NADH-GDH. |
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Keywords: | 5 5′ -dithio-bis (2-nitrobenzoic acid) dithiotreitol Zea mays |
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