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| 黄芩有效成分提取及对炎症反应的影响 | |
| 引用本文： | 朱伟,张守勤,孙红光,朱迅. 黄芩有效成分提取及对炎症反应的影响[J]. 生物加工过程, 2009, 7(1): 69-73 |
| 作者姓名： | 朱伟 张守勤 孙红光 朱迅 |
| 作者单位： | 1. 海南大学,海洋学院制药工程系,海南省热带水生生物技术重点实验室,海口,570228 2. 吉林大学,生物与农业工程学院,长春,130025 3. 吉林大学,基础医学院免疫教研室,长春,130021 |
| 基金项目： | 国家自然科学基金，海南省自然科学基金，海南大学自然科学基金 |
| 摘 要： | 利用常温超高压方法提取黄芩中黄酮类物质，得到黄芩甲醇提取物（SBM）。对该物质抗炎作用研究发现SBM（31．25-500mg／L）可抑制刀豆蛋白A（ConA）诱导的小鼠脾淋巴细胞增殖和脂多糖（LPS）诱导的腹腔巨噬细胞TNFα合成，SBM（125mg／L）的抑制率分别可达96％、99％；SBM（10mg／kg、20mg／kg）也可抑制大鼠棉球性肉芽肿，但不影响IKBα蛋白磷酸化。常温超高压方法可有效提取黄芩中黄酮类物质，黄芩提取物SBM可通过抑制淋巴细胞功能、炎症介质发挥抗炎作用。 |
| 关 键 词： | 炎症 IKBα 黄芩 TNFα |
Separation of Scutellaria baicalensis and its anti-inflammatory activity |
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| ZHU Wei,ZHANG Shou-qin,SUN Hong-guang,ZHU Xun. Separation of Scutellaria baicalensis and its anti-inflammatory activity[J]. Chinese Journal of Bioprocess Engineering, 2009, 7(1): 69-73 | |
| Authors: | ZHU Wei ZHANG Shou-qin SUN Hong-guang ZHU Xun |
| Affiliation: | 1. Department of Pharmaceutical Engineering, Hainan Key Laboratory of Tropical Hydrobiological Technology, Oceanology College, Hainan University, Haikou 570228, China; 2. College of Biology and Agricultural Engineering, Jilin University, Changchun 130025, China; 3. Department of Immunology, School of Basic Medical Sciences, Jilin University,Changchun 130021, China) |
| Abstract: | The extract-SBM was isolated from Scutellariae radix by high hydrostatic pressure. The effects of SBM on ConA-induced splenocyte proliferation, LPS-induced TNFα production, and protein phosphorylation of IκBα were studied in vitro, and the therapeutic effects of SBM on cotton pellet-induced rat granuloma was also investigated. SBM （31.25-500 mg/L） inhibited ConA-induced splenocyte proliferation in mice. SBM （31.25-250 rag/L） inhibited LPS-induced TNFα production. Cotton pellet-induced rat granuloma was significantly decreased by SBM with the doses of 10 mg/kg and 20 mg/kg. SBM could inhibit inflammatory reaction. Its mechanisms were related to the suppression of inflammatory medium production. High hydrostatic pressure treatment could been used to separate the medical plant. |
| Keywords: | inflammation IκBα ScuteUaria baicalensis TNFα |
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