Proteomic analysis of endogenous conjugated linoleic acid biosynthesis in lactating rats and mouse mammary gland epithelia cells (HC11) |
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| Authors: | Yong-Cheng Jin Hong-Gu Lee Cheng-Xiong Xu Jeng-A Han Seong-Ho Choi Man-Kang Song Young-Jun Kim Ki-Beom Lee Seon-Ku Kim Han-Seok Kang Byung-Wook Cho Teak-Soon Shin Yun-Jaie Choi |
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| Institution: | 1. Laboratory of Animal Cell Biotechnology, Department of Agricultural Biotechnology, Seoul National University, Seoul 157-921, Republic of Korea;2. Department of Animal Science and PNU-Special Animal Biotechnology Center, Pusan National University, Miryang, Gyeongnam 627-706, Republic of Korea;3. Department of Hematology and Medical Oncology, Winship Cancer Institute, Emory University School of Medicine, Atlanta, GA 30322, USA;4. Department of Animal Science, ChungBuk National University, Cheong-Ju, ChungBuk, Republic of Korea;5. Department of Food Science, Korea University, Seoul 136-701, Republic of Korea;6. Department of Biotechnology, SongDo Techno Park, 7-50 Songdo-Dong, Yeonsu-Gu, Incheon 406-840, Republic of Korea |
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| Abstract: | This study was conducted to investigate the amount of CLA synthesized endogenously by rat mammary tissues in response to TVA (a precursor for cis-9, trans-11 CLA endogenous synthesis) treatment as well as the differences in the protein expression of genes encoding the biosynthesis of CLA in rat mammary tissue and mouse mammary gland epithelia cells (HC11). Treatment with TVA resulted in improved CLA productivity. Furthermore, 2-DE revealed two spots in samples of mammary tissues and one spot in samples of mammary gland epithelia cells (HC11) that were consistently altered in the TVA treatment groups when compared with the control group (non-fatty acid). The mRNA expression patterns of three of the proteins (PDI, PRDX2, LAMR1), as measured by real-time PCR, were similar to the pattern of protein abundance. In addition, the expression of SCD mRNA in the mammary tissue of rats and HC11 cell treated with TVA was higher than in the control group. Our results suggest that the identified proteins may be related to CLA biosynthesis in mammary tissue. |
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| Keywords: | CLA conjugated linoleic acid TVA trans vaccenic acid PDI protein disulfide-isomerase precursor PRDX2 peroxiredoxin-2 LAMR1 laminin receptor 1 SCD stearoyl-CoA desaturase FAS fatty acid synthase LPL lipoprotein lipase |
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