Different sample treatment approaches for the analysis of T-2 and HT-2 toxins from oats-based media |
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Authors: | Angel Medina Francisco M Valle-Algarra Misericordia Jiménez Naresh Magan |
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Institution: | 1. Applied Mycology Group, Cranfield Health, Vincent Building, Cranfield University, College Road, Cranfield, Bedfordshire, MK43 0AL, UK;2. Departamento de Microbiología y Ecologia, Universitat de Valencia, Dr. Moliner 50, 46100 Burjassot, Valencia, Spain |
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Abstract: | A LC-DAD method is proposed for the determination of the T-2 and HT-2 toxins in cultures of Fusarium langsethiae in oat-based and other in vitro media. Test media consisted of freshly prepared milled oats to which T-2 and HT-2 toxin stock solutions were added. Different mixtures of extraction solvent (acetonitrile:water and methanol:water), extraction times (30′, 60′ or 90′) and drying methods were investigated. Results showed that extraction with methanol:water (80:20, v/v) for 90 min, drying with N2 and subsequent analysis by LC-DAD was the fastest and most user friendly method for detecting HT-2 and T-2 toxins production by F. langsethiae strains grown on oat-based media at levels of 0.459 and 0.508 mg of toxin/kg of agar, respectively. The proposed method was used to investigate toxin production of 6 F. langsethiae strains from northern Europe and provided clear chromatograms with no interfering peaks in media with and without glycerol as water activity modifier. |
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Keywords: | Analysis Type A trichothecenes Diode array Cereals |
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