Elastic incoherent neutron scattering as a probe of high pressure induced changes in protein flexibility |
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| Authors: | A Filabozzi A Deriu MT Di Bari D Russo S Croci A Di Venere |
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| Institution: | 1. Università di Roma “Tor Vergata”, Dipartimento di Fisica and CNISM, Roma, Italy;2. Università di Parma, Dipartimento di Fisica and CNISM, , Parma, Italy;3. CNR-INFM, CRS-SOFT, Institut Laue-Langevin, Grenoble, France;4. Università di Parma, Dipartimento di Sanità Pubblica, CNISM and INBB, Parma, Italy;5. Università di Roma “Tor Vergata”, Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Roma, Italy |
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| Abstract: | We report here the results of elastic incoherent neutron scattering experiments on three globular proteins (trypsin, lysozyme and β-lactoglobulin) in different pressure intervals ranging from 1 bar to 5.5 kbar. A decrease of the mean square hydrogen fluctuations, 〈u2〉, has been observed upon increasing pressure. Trypsin and β-lactoglobulin behave similarly while lysozyme shows much larger changes in 〈u2〉. This can be related to different steps in the denaturing processes and to the high propensity of lysozyme to form amyloids. Elastic incoherent neutron scattering has proven to be an effective microscopic technique for the investigation of pressure induced changes in protein flexibility. |
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| Keywords: | Neutron scattering High pressure Protein unfolding Protein flexibility Trypsin Lysozyme Beta-lactoglobulin |
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