Electrochromatographic characterization of methacrylate ester-based monolith and capillary electrochromatography separation of flavonoids |
| |
Authors: | Zongbao Chen Yan Cai Jintian Cheng Lan Zhang |
| |
Affiliation: | 1. Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian, China;2. The Sport Science Research Center, Fuzhou University, Fuzhou 350002, Fujian, China;3. Department of Chemistry, Shangrao Normal University, 334001, Jiangxi, China |
| |
Abstract: | A porous polymethacrylate ester-based monolithic column for capillary electrochromatography (CEC) was designed by mean of in situ co-polymerizing lauryl methacrylate (LMA), ethylene dimethacrylate (EDMA) and 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS) in a ternary porogenic solvent including cyclohexanol, 1,4-butanediol and water. After investigating the influence factors of the CEC monolithic columns, four flavonoids (i.e., Rutin, Quercetin, Kaempferol, and Quercitrin) were separated and assayed to evaluate this monolithic column with CEC method. Under optimum conditions, the CEC method exhibited high separation efficiency, with rapid separation time of 3–4 min, for the four flavonoid samples using 10 mM phosphate buffer containing 70% acetonitrile (pH 9.0). Importantly, the proposed method could provide a promising approach for rapid separation and detection in biomedicine. |
| |
Keywords: | Capillary electrochromatography Flavonoids Monolithic columns |
本文献已被 ScienceDirect 等数据库收录! |
|