Effects of defined synthetic phospholipids on glycosylation processes. Modifications of membrane-bound N-acetylglucosaminyl-transferase and solubilized sialyl-transferase activities |
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Authors: | H Bador O Gateau P Louisot R Morelis |
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Institution: | Biology Division, Oak Ridge National Laboratory, Oak Ridge, TN 37830, U.S.A. |
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Abstract: | In cultures of hamster embryo cells, benzoa]pyrene (Ba]P) is metabolized primarily in the bay region. In contrast, little or no bay region metabolism of the noncarcinogenic isomer benzoe]pyrene (Be]P) could be detected during 12–96-h incubations of hamster embryo cells with 4 μM 3H]Be]P. The upper limit to 9,10-dihydro-9,10-dihydroxy-Be]P formation is about 0.2% of the ethyl acetate-soluble metabolites ( <0.1% of the total metabolites). The major identified metabolites of Be]P were 4,5-dihydro-4,5-dihydroxy Be]P and the glucuronide conjugates of 3-OH-Be]P and 4,5-dihydro-4,5-dihydroxy Be]P. Simultaneous treatment of cells with either Ba]P or 7,8-benzoflavone (BF) did not induce bay region metabolism of 3H]Be]P. |
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Keywords: | MES methylethane sulphonate |
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