The determination of 2'-O-methylnucleosides in RNA |
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| Authors: | L M Pike F Rottman |
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| Institution: | Department of Biochemistry, Michigan State University, East Lansing, Michigan 48824 USA |
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| Abstract: | A rapid and sensitive procedure is described for determining the 2′-O-methylnucleoside methylnucleoside composition of an RNA sample. The RNA is enzymatically hydrolyzed to nucleosides and the 2′-O-methylnucleoside fraction is isolated by DEAE-cellulose (borate) column chromatography. Boric acid is removed as its methyl ester and the 2′-O-methylnucleosides are resolved by liquid chromatography in the presence of ethylene glycol. The sensitivity of this method is sufficient to distinguish RNA samples which differ only 2–3% in 2′-O-methylnucleoside composition. |
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| Keywords: | To whom to address correspondence |
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