Genebank biodiversity assessments regarding optimal sample size and seed harvesting techniques for the regeneration of carrot accessions |
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Authors: | Valerie Le Clerc Mathilde Briard Jacky Granger Jerome Delettre |
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Affiliation: | (1) Northwest Indian Fisheries Commission, 6730 Martin Way E., Olympia, WA 98516, USA;(2) Present address: School of Aquatic and Fishery Sciences, University of Washington, Seattle, WA 98195, USA;(3) Stillaguamish Tribe, P.O. Box 277, Arlington, WA 98223, USA |
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Abstract: | Small, finite populations are particularly vulnerable to diversity loss during regeneration. The regeneration of a highly outbreeding open-pollinated variety relies on estimated effective population size, via the measurement of temporal change in allele frequencies. Using appropriate estimators for dominant gene markers, effective sizes were calculated for five sizes of a mating population and two seed harvesting procedures. We have shown that, in the case of carrot regeneration, 70 equally harvested plants should provide an effective size (Ne) of at least 50 plants. This value seems sufficient to limit genetic drift and to preserve an efficient level of genetic diversity within the collection. The efficiency of balanced samples (made of an equal number of seeds per plant) is compared to that of bulk samples (seeds randomly chosen among the total seed lot coming from all the plants). |
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Keywords: | Bulk/balanced samples Daucus carota L. Ex situ conservation Genetic drift Seed collections |
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