Isolation of highly polymorphic microsatellite markers from the intertidal barnacle Chthamalus montagui Southward |
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Authors: | FEDERICA G. PANNACCIULLI SANIT PIYAPATTANAKORN JOHN D. D. BISHOP STEPHEN J. HAWKINS NORMAN MACLEAN |
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Abstract: | This study reports the isolation and characterization of seven highly polymorphic microsatellite loci in Chthamalus montagui (Crustacea, Cirripedia). The loci were isolated from a library constructed from genomic DNA enriched for CA repeats. The markers yielded three to 43 alleles per locus (mean 16.7) in samples averaging 49 individuals. Observed heterozygosity ranged from 0.08 to 0.58 (mean 0.39). These microsatellite loci will be valuable tools for population genetic studies of this species. |
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Keywords: | barnacle Chthamalus montagui microsatellites PCR |
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