Isolation of 12 polymorphic microsatellite loci in the phytopathogenic fungus Alternaria brassicicola |
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Authors: | HERV AVENOT,ANITA DONGO,NELLY BATAILL ‐SIMONEAU,B ATRICE IACOMI‐ VASILESCU,BRUNO HAMON,DIDIER PELTIER,PHILIPPE SIMONEAU |
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Affiliation: | HERVÉ AVENOT,ANITA DONGO,NELLY BATAILLÉ‐SIMONEAU,BÉATRICE IACOMI‐ VASILESCU,BRUNO HAMON,DIDIER PELTIER,PHILIPPE SIMONEAU |
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Abstract: | Twelve polymorphic microsatellite markers were isolated from the phytopathogenic fungus Alternaria brassicicola, the causal agent of black spot of crucifers. An enrichment protocol was used to isolate microsatellite loci, which were then analysed in a collection of 46 isolates sampled from seven different countries. The number of alleles detected in 12 loci ranged from two to 10 (mean 3.5). Investigation of cross‐species amplifications showed that the designed primers were specific to A. brassicicola. |
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Keywords: | Alternaria brassicicola crucifers genetic diversity microsatellite |
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