Conservation and versatility of a new set of primers for long‐PCR amplification of complete insect mitochondrial genomes based on Haematobia irritans mtDNA sequences |
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Authors: | JOAN GRANDE BARAU,ANA MARIA LIMA DE AZEREDO‐ESPIN,ANA CL UDIA LESSINGER |
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Affiliation: | JOAN GRANDE BARAU,ANA MARIA LIMA DE AZEREDO‐ESPIN,ANA CLÁUDIA LESSINGER |
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Abstract: | The amplification of complete mitochondrial genomes by long PCR (polymerase chain reaction) has been a major contribution to the large‐scale sequencing of arthropodan mitochondrial genomes. In this work, we designed six conserved long‐PCR primers to successfully recover the entire mitochondrial genome of the horn fly Haematobia irritans (Diptera: Muscidae) in two overlapping fragments. The conservation and versatility of these primers were tested for 17 other species from four major insect orders: Diptera (14), Coleoptera (1), Lepidoptera (1) and Hymenoptera (1). The amplification of complete mitochondrial genomes in orders other than Diptera suggested an even broader application of these primers, especially within the Hexapoda. |
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Keywords: | Haematobia irritans insects long PCR mitochondrial genome primers |
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