Characterization and PCR multiplexing of polymorphic microsatellite loci for the locust Locusta migratoria |
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| Authors: | MARIE‐PIERRE CHAPUIS ANNE LOISEAU YANNIS MICHALAKIS MICHEL LECOQ ARNAUD ESTOUP |
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| Affiliation: | 1. Centre de Biologie et de Gestion des Populations, INRA, Campus International de Baillarguet CS 30016, 34988 Montferrier/Lez, France,;2. Centre d’Etudes sur le Polymorphisme des Microorganismes, UMR CNRS‐IRD 9926, Montpellier cedex 5, France,;3. Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Campus International de Baillarguet 34398 Montpellier cedex 5, France;4. Centre de Biologie et de Gestion des Populations, INRA, Campus International de Baillarguet CS 30016, 34988 Montferrier/Lez, France, Arnaud Estoup, Fax: +33 (0)499623345;5. E‐mail: |
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| Abstract: | Because of the scarcity of polymorphic genetic markers available in locust species, only a few population genetics studies have been carried out on this taxon. We isolated and characterized 11 polymorphic microsatellite loci in the pest locust Locusta migratoria capito, and described experimental conditions for polymerase chain reaction (PCR) multiplexing and simultaneously genotyping these loci. The number of alleles per locus ranged from six to 25, and the expected heterozygosity ranged from 0.431 to 0.957. Results of cross‐taxon amplification tests are reported in six other Locusta migratoria subspecies, six species of the Oedipodinae subfamily and two other pest locust species. |
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| Keywords: | locust Locusta migratoria microsatellites null alleles Orthoptera pest |
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