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Development and characterization of microsatellite markers for two dioecious Ficus species
Authors:MONIKA ZAVODNA  PAUL ARENS  PETER J. VAN DIJK  BEN VOSMAN
Abstract:Microsatellite markers for Ficus montana and Ficus septica were developed using genomic libraries enriched for di‐, tri‐ and tetranucleotide repeats. The subsets of five and three best scorable primer pairs were characterized on 24 F. montana and 36 F. septica individuals, respectively. For F. montana, loci showed five to 14 alleles per locus and expected heterozygosities ranged between 0.23 and 0.87. For F. septica, loci showed three to five alleles per locus and expected heterozygosities ranged between 0.36 and 0.49. Four primer pairs (two from each subset) cross‐amplified in the other species, indicating transportability of the markers within the genus Ficus.
Keywords:dispersal  Ficus montana  Ficus septica  mutualism  pollinating fig wasps  simple sequence repeat (SSR)
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