Colorimetric determination of L-phenylacetyl carbinol produced by biotransformation of benzaldehyde and pyruvate using Saccharomyces cerevisiae |
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| Authors: | P Nikolova A Long O P Ward |
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| Institution: | (1) Department of Biology, University of Waterloo, N2L 3G1 Waterloo, Ont., Canada;(2) Trinity College Medical School, St. James's Hospital, Dublin, Ireland |
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| Abstract: | Summary The utility of a colorimetric method, based on the Voges-Proskauer reaction, for L-phenylacetyl carbinol (L-PAC) determination has been evaluated. The relative absorbances observed for equivalent concentrations of L-PAC and acetylbenzoyl at 580 nm were determined. L-PAC manifested a lower absorbance (60–68%) than the equivalent acetylbenzoyl concentration. |
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