Influence of indole-butyric acid and electro-pulse on in vitro rooting and development of olive (<Emphasis Type="Italic">Olea europea</Emphasis> L.) microshoots |
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Authors: | Isabel Maria Gonzalez Padilla I Vidoy C L Encina |
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Institution: | (1) Estación Experimental La Mayora, CSIC, Algarrobo-Costa, Málaga, Spain;(2) Present address: IFAPA, Churriana, Málaga, Spain |
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Abstract: | The effects of indole-butyric acid (IBA) and electro-pulses on rooting and shoot growth were studied in vitro, using olive
shoot cultures. Tested shoots were obtained from seedlings belonging to three Spanish cultivars, ‘Arbequina’, ‘Manzanilla
de Sevilla’ and ‘Gordal Sevillana’, which have easy-, medium- and difficult-to-root rooting abilities, respectively. The standard
two-step rooting method (SRM), consisting of root induction in olive rooting medium supplemented with 0, 0.1 or 1 mg/l IBA
followed by root elongation in the same rooting medium without IBA, was compared with a novel one-step method consisting of
shoot electro-pulses of 250, 1,250 or 2,500 V in a solution of IBA (0, 0.1 or 1 mg/l) and direct transferral to root elongation
medium. The rooting percentage of the seedling-derived shoots obtained with the SRM was 76% for ‘Arbequina’ and ‘Gordal Sevillana’
cultivars and 100% for ‘Manzanilla de Sevilla’ cultivar, whereas with the electro-pulse method, the rooting percentages were
68, 64 and 88%, respectively. IBA dipping without pulse produced 0% rooting in ‘Arbequina’ seedling-derived shoots. The electroporation
in IBA not only had an effect on shoot rooting but also on shoot growth and development, with longer shoots and higher axillary
shoot sprouting and growth after some of the treatments. These effects were cultivar-dependent. The electro-pulse per se could
explain some of these effects on shoot development.
I.M.G. Padilla and I. Vidoy contributed equally to this article. |
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Keywords: | Arbequina Gordal Sevillana Manzanilla de Sevilla Seedlings Electroporation |
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