Role of ecdysone 20-monooxygenase in regulation of 20-hydroxyecdysone levels by juvenile hormone and biogenic amines in Drosophila |
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Authors: | Inga Yu. Rauschenbach N. E. Gruntenko N. A. Chentsova N. V. Adonyeva A. A. Alekseev |
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Affiliation: | (1) Institute of Cytology and Genetics, Siberian Division of Russian Academy of Sciences, Lavrentjev ave., 10, Novosibirsk , 630090, Russia;(2) Institute of Chemical Kinetics and Combustion, Siberian Division of Russian Academy of Sciences, Novosibirsk , 630090, Russia |
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Abstract: | The effects of increased levels of dopamine (feeding flies with dopamine precursor, l-dihydroxyphenylalanine) and octopamine (feeding flies with octopamine) on ecdysone 20-monooxygenase activity in young (2 days old) wild type females (the strain wt) of Drosophila virilis have been studied. l-dihydroxyphenylalanine and octopamine feeding increases ecdysone 20-monooxygenase activity by a factor of 1.6 and 1.7, respectively. Ecdysone 20-monooxygenase activity in the young (1 day old) octopamineless females of the strain Tβh nM18 , in females of the strain P845 (precursor of Tβh nM18 strain) and in wild type females (Canton S) of Drosophila melanogaster have been measured. The absence of octopamine leads to a considerable decrease in the enzyme activity. We have also studied the effects of juvenile hormone application on ecdysone 20-monooxygenase activity in 2-day-old wt females of D. virilis and demonstrated that an increase in juvenile hormone titre leads to an increase in the enzyme activity. We discuss the supposition that ecdysone 20-monooxygenase occupies a key position in the regulation of 20-hydroxyecdysone titre under the conditions that lead to changes in juvenile hormone titre and biogenic amine levels. |
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Keywords: | Drosophila Ecdysone 20-monooxygenase Dopamine Octopamine Juvenile hormone |
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