Modifications of lysosomal enzymes in Dictyostelium discoideum |
| |
Authors: | Hudson H. Freeze |
| |
Affiliation: | (1) Cancer Biology Program, Cancer Center, University of California, San Diego, 7-012, 92093 La Jolla, CA, USA |
| |
Abstract: | This paper has two purposes. The first is to review the past studies on the structure, biosynthesis, and immunological properties of a class of glycoproteins, the lysosomal enzymes, in Dictyostelium discoideum. The second purpose is to present new data on the analysis of mutant strains altered in the biosynthesis of the lipid-linked precursor of N-linked oligosaccharides, and on the characterization of new carbohydrate antigenic determinants found on multiple proteins in Dictyostelium. We will also show how a combination of genetic, biochemical and immunochemical approaches have been used to unravel a portion of the glycosylation pathway in Dictyostelium.The long-term goal of these studies is to use Dictyostelium discoideum as a model system to understand the functions of a variety of glycoconjugates in a multicellular organism. The existence of a large number of mutant strains which are altered in a variety of cellular functions, development and the posttranslational modification of multiple proteins, offers a great opportunity to explore this area. |
| |
Keywords: | Dictyostelium discoideum lysosomal enzymes glycosylation sulfated N-linked oligosaccharides common antigens |
本文献已被 SpringerLink 等数据库收录! |
|