Light and electron microscopical immunohistochemical localization of large proteoglycans in human tooth germs at the bell stage |
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Authors: | Kiyoshi Yamada Toshihiro Yamada Takahisa Sasaki Firoz Rahemtulla Minoru Takagi |
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Institution: | (1) Department of Periodontology, Nihon University School of Dentistry, 1-8-13, Kanda-Surugadai, Chiyoda- ku Tokyo, 101;(2) Department of Anatomy, Nihon University School of Dentistry, 1-8-13, Kanda-Surugadai, Chiyoda- ku Tokyo, 101;(3) Department of Oral Anatomy, School of Dentistry, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142, Japan;(4) Department of Oral Biology, University of Alabama at Birmingham, 740 School of Dentistry Building, 1919 Seventh Avenue South, UAB Station, Birmingham, Alabama 35294-0007, USA |
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Abstract: | Summary The immunohistochemical localization of large hyaluronate-binding proteoglycans has been studied in human tooth germs at the
bell stage using a monoclonal antibody, 5D5, which is derived from bovine sclera and specifically recognizes the core protein
of large proteoglycans, such as versican, neurocan and brevican, but not that of aggrecan. In the early bell stage before
predentine secretion, when the enamel organs consisted of the inner and outer enamel epithelia, stratum intermedium and stellate
reticulum, the enamel organs were not stained by 5D5, but the dental papillae and follicles stained strongly. Concomitant
with the secretion of predentine, dentine and subsequent enamel matrix, strong 5D5 immunostaining distributed over the entire
cell surfaces of secretory ameloblasts was observed. The forming enamel matrix showed strong staining. While most of the inner
and outer enamel epithelia and stratum intermedium lacked staining, the cervical loop region and stellate reticulum showed
weak staining. Although the forming dentine and odontoblasts appeared to lack 5D5 affinity, the predentine, dental papilla
and dental follicle demonstrated moderate to strong reactivity. At the ultrastructural level, specific immunoreaction by immunogold
particle deposition was clearly detected over the basal lamina of presecretory ameloblasts, secretion granules of secretory
ameloblasts and the forming enamel matrix. These results indicate that a marked increase in the large proteoglycan associated
with secretory ameloblasts may correlate with cell differentiation and enamel matrix biosynthesis.
This revised version was published online in November 2006 with corrections to the Cover Date. |
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