Department of Biochemistry, Guy's Hospital Medical School, London SE1 9RT, U.K.;Professorial Unit of Skin Pharmacology and Department of Bacteriology, Institute of Dermatology, Homerton, London E9 6BX, U.K.
Abstract:
Abstract The metabolism of testosterone by coryneform bacteria in vitro has been studied. Metabolites identified after derivatization by capillary gas chromatography and further by combined gas chromatography-mass spectrometry were 17β-hydroxy-5α-androstan-3-one and 17β-hydroxy-5β-androstan-3-one. The mass spectral characteristics of the methyl oxime trimethylsilyl ethers of all the 17-hydroxy-androstan-3-one and 3-hydroxy-androstan-17-one isomers are recorded.