Reidentification of the keratinase-producing facultatively alkaliphilic Bacillus sp. AH-101 as Bacillus halodurans |
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| Authors: | Hideto Takami Yuichi Nogi Koki Horikoshi |
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| Affiliation: | (1) Deep-sea Microorganisms Research Group, Japan Marine Science and Technology Center, 2-15 Natsushima, Yokosuka 237-0061, Japan Tel. +81-468-67-3895; Fax +81-468-66-6364 e-mail: takamih@jamstec.go.jp, JP |
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| Abstract: | Alkaliphilic Bacillus sp. AH-101 was characterized in terms of physiological and biochemical characteristics, and 16S rDNA sequence homology and DNA–DNA hybridization analyses were performed. Phylogenetic analysis of strain AH-101 based on comparison of 16S rDNA sequences revealed that this strain is closely related to Bacillus halodurans. DNA–DNA hybridization of AH-101 and related Bacillus reference strains showed that the highest level of DNA–DNA relatedness (88%) was found between strain AH-101 and the B. halodurans type strain (DSM497). Our findings demonstrate that strain AH-101 is a member of the species B. halodurans. Received: June 10, 1999 / Accepted: August 6, 1999 |
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| Keywords: | Alkaliphilic Bacillus halodurans AH-101 DNA– DNA hybridization Phylogenetic tree Identification |
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