The major soluble cytochromes of the obligately aerobic sulfur bacterium,Thiobacillus neapolitanus |
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| Authors: | P A Trudinger T E Meyer R G Bartsch M D Kamen |
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| Institution: | (1) Chemistry Department, University of California, 92093 San Diego, CA, USA;(2) Present address: Baas Becking Geobiological Laboratory, P.O. Box 378, 2601 Canberra City, A.C.T., Australia;(3) Present address: Biochemistry Department, University of Arizona, 85721 Tucson, AZ, USA |
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| Abstract: | Four cytochromes were isolated from soluble extracts of the aerobic sulfur bacterium, Thiobacillus neapolitanus. The two most abundant proteins were purified to homogeneity and thoroughly characterized. Cytochrome c-554 (547) is a monomeric, small molecular weight protein which is unusual in having two well-resolved alpha peaks in UV-visible absorption spectra. The redox potential is 208 mV. Native cytochrome c-549 is oligometric, but has a subunit size of about 26.000. The yield of this protein could be improved dramatically by washing membranes with 30% ammonium sulfate, but the material solubilized by this method had a larger native molecular weight than that in the initial 0.1 M Tris-Cl extract and behaved differently on chromatography. The properties of cytochrome c-549 including subunit size and UV-visible absorption spectra are similar to mitochondrial cytochrome c
1 and chloroplast cytochrome f, which suggests that it may be a modified form of the predominant membrane cytochrome. Based on cytochrome content, it is suggested that T. neapolitanus is not closely related to other thiobacilli.Dedicated to Prof. Dr. G. Drews on the occasion of his sixtieth birthday |
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| Keywords: | Bacterial sulfur oxidation Cytochromes c Bacterial taxonomy Electron transfer proteins |
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