Pilot-scale production of carboxymethylcellulase from rice hull by Bacillus amyloliquefaciens DL-3 |
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| Authors: | Kang-Ik Jo You-Jung Lee Bo-Kyung Kim Bo-Hwa Lee Chung-Han Chung Soo-Wan Nam Sung-Koo Kim Jin-Woo Lee |
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| Institution: | (1) Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University, Busan, 604-714, Korea;(2) Department of Biotechnology and Bioengineering, College of Engineering, Dong-Eui University, Busan, 614-714, Korea;(3) Department of Biotechnology and Bioengineering, College of Fisheries Science, Pukyung National University, Busan, 608-737, Korea |
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| Abstract: | Optimal conditions for pilot-scale production of the carboxymethylcellulase (CMCase) by Bacillus amyloliquefaciens DL-3 were investigated. The best carbon and nitrogen sources for the production of CMCase by B. amyloliquefaciens DL-3 were found to be rice hull and peptone and their optimal concentrations were 5.0 and 0.20% (w/v), respectively. Optimal
temperature and initial pH for the production of CMCase were 37°C and 6.8. Optimal agitation speed and aeration rate for the
production of CMCase were 300 rpm and 1.0 vvm in a 7 L bioreactor, which were different from those for the cell growth of
B. amyloliquefaciens DL-3. The highest productions of CMCase by B. amyloliquefaciens DL-3 from 5.0% (w/v) rice hull as a carbon source under optimal conditions in a 7 or 100 L bioreactor were 220 and 367 U/mL,
respectively. |
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| Keywords: | Bacillus amyloliquefaciens carboxymethylcellulase (CMCase) optimization rice hull |
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