Identification, sequence, and expression of Treponema denticola mcpA, a putative chemoreceptor gene |
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| Authors: | Shermalyn R Greene Lola V Stamm |
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| Institution: | Department of Biochemistry, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto 602, Japan;Department of Biotechnology, Faculty of Engineering, Osaka University, 2-1 Yamadaoka, Suita-shi, Osaka 565, Japan |
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| Abstract: | The nucleotide sequence of a methyl-accepting chemotaxis protein gene, mcpA, from Treponema denticola has been determined. The mcpA gene encodes a 729-amino acid protein whose deduced amino acid sequence has significant homology with several bacterial MCPs. T. denticola McpA contains two N-terminal transmembrane regions and two C-terminal putative methylation sequences that are separated by a highly conserved signaling domain. The organization of these structural features is characteristic of MCPs. The observed molecular mass of the in vitro synthesized McpA (76.0 kDa) correlates with the predicted molecular mass of the protein (80.1 kDa). |
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| Keywords: | Constitutive mutant Negative regulation Thiamine metabolism Yeast |
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